Gerald M. Borromeo,
Jessie C. Roxas,
Stevenson S. Sayson
College of Medical Laboratory Science - Our Lady of Fatima University
Medical Laboratory Science
The water is one of the most important necessities of man, without water all living things will perish. And as a major need, water is used extensively in our everyday lives, like consumption for drinking, cleaning, as solvent for industrial use and other household purposes.
This study used the experimental research design. This study was conducted to determine the effectivity of Sechium edule (chayote) leaves extract in lowering the bacterial count of the water sample obtained from the wawa river for the household use of dumagat compared with the control groups. The experimental utilized leaves extract of Sechium edule. Water samples were collected at the riverside of the wawa river where the dumagat people get their household water. The water samples were tested first to check if there were bacteria in the water sample using mac conkey agar and blood agar plated media, before proceeding to the Main experiment.
The positive control were treated with one milliliter of chlorine per liter of water, and the experimental group were treated with Sechium edule leaves extract, while the negative control was uesd without treatment after preparation of the water samples, water bacteriology was done using dilutions 1:10, 1:100, 1:1000 to assess the number of bacteria growing on each plates having the positive, negative and experimental groups.
The computed means were 243.666 cfu/ml for experimental and 340.333 for the negative control, based on the computed means, the researchers assumed that the bacterial colony count for the following locations of upstreams, midstream and downstream showed lowering of bacterial counts, compared to the negative control, to check for the null hypothesis, the researchers computed P value for the following locations, for upstream was .08, for the midstream was .14 and for the downstream was 0.03, which is based on the .05 alpha value, which indicates if the result were significant or not significant, higher value than .05 indicates no significance and lower than .05 indicates significant difference, on the following result, it showed that upstream bacterial colony count and midstream colony count showed no significant difference and the downstream bacterial showed significant difference, indicating a positive result for the downstream bacterial colony count.
This study wanted to determine the effectivity of Sechium edule leves extract in lowering the bacterial count of the water sample from Wawa river for household use.
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