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Effectivity of Sechium edule (Chayote) leaves extract in lowering the bacterual count of Wawa river for household use of Dumagat people.


Gerald M. Borromeo,
Jessie C. Roxas,
Stevenson S. Sayson

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College of Medical Laboratory Science - Our Lady of Fatima University

Publication Information

Publication Type
Medical Laboratory Science
Publication Date


The water is one of the most important necessities of man, without water all living things will perish. And as a major need, water is used extensively in our everyday lives, like consumption for drinking, cleaning, as solvent for industrial use and other household purposes.

This study used the experimental research design. This study was conducted to determine the effectivity of Sechium edule (chayote) leaves extract in lowering the bacterial count of the water sample obtained from the wawa river for the household use of dumagat compared with the control groups. The experimental utilized leaves extract of Sechium edule. Water samples were collected at the riverside of the wawa river where the dumagat people get their household water. The water samples were tested first to check if there were bacteria in the water sample using mac conkey agar and blood agar plated media, before proceeding to the Main experiment.

The positive control were treated with one milliliter of chlorine per liter of water, and the experimental group were treated with Sechium edule leaves extract, while the negative control was uesd without treatment after preparation of the water samples, water bacteriology was done using dilutions 1:10, 1:100, 1:1000 to assess the number of bacteria growing on each plates having the positive, negative and experimental groups.

The computed means were 243.666 cfu/ml for experimental and 340.333 for the negative control, based on the computed means, the researchers assumed that the bacterial colony count for the following locations of upstreams, midstream and downstream showed lowering of bacterial counts, compared to the negative control, to check for the null hypothesis, the researchers computed P value for the following locations, for upstream was .08, for the midstream was .14 and for the downstream was 0.03, which is based on the .05 alpha value, which indicates if the result were significant or not significant, higher value than .05 indicates no significance and lower than .05 indicates significant difference, on the following result, it showed that upstream bacterial colony count and midstream colony count showed no significant difference and the downstream bacterial showed significant difference, indicating a positive result for the downstream bacterial colony count.


This study wanted to determine the effectivity of Sechium edule leves extract in lowering the bacterial count of the water sample from Wawa river for household use.


1. Anima, N. , Yuri, M. . Indigenous People of Philippines # 971 Capp. Street, San Francisco : Philippine Library Congress Cataloguing., 2011. 7-8.
2. Brown, W. H.. Useful Plants of the Philippines Manila : Bureau of Science, 1950. 397-398.
3. Binnie, C. , Kimber, M. , Smethurst, G. . Basic water treatment London: Thomas Telford publishing, 2002. 11.
4. Diré, G. F., Coura, M. C., Almeida, M. L., Vasconcelos, S. D., Siqueira, P. A., Duarte, R. M., Bernardo-Fihlo, M. M.. "Study of the Effects of a Chayotte (Sechium edule) Extract on the Plasmid Puc. 9.1 DNA" Plant Pathology Journal 6(1): 72-76, 2007
5. Eaton, A. D., Clesceri, L. S., Rice, E. W., Greenberg, A. E.. Standard Methods For Examination Of Water And Waste Water Washington, District of Columbia: American Public Health Association, 2005. 5-9.
6. Gray, N. F.. Water technology China: Iwa Publishing house, 2010. 360.
7. Hammer, M. J.. Water and Waste Water Technology 5th Edition Jurong Singapore: Pearson Education South Asia Pte Ltd. , 2005.
8. Lim, T. K.. Edible Medicinal and Non- Medicinal Plants: Volume 1, Fruits. New York City: Springer Science + Business Media B.V, 2012. 384.
9. Nuevo, J. J. M., Bernardino, E. R. H.. Laboratory Manual in Medical Bacteriology Our Lady of Fatima University,
10. Polunin, N. V.C.. Aquatic ecosystem USA: Cambridge University Press, 2008. 149-153.
11. Van Wyle, B. E.. Food Plants of the World South Africa : Briza Publications Inc, 2005. 343.
12. Peace Corp’s, Information Collection and Exchange. Water purification, Distribution and sewage disposal for peace corps volunteer. Volunteer For International Technical Assistance, Peace Corp’s 2009. 15.
13. Westcoat, J. L., White, G. F.. Water for Life: Water Management and Environmental Policy The Pitt Building, Trumpington Street, Cambridge, United Kingdom: Cambridge University Press , 2003. 22.
14. Baguma, D. , Loiskandl, W. , Darnhofer, I. , Jung, H. , Hauser, M. . "Knowledge of measures to safeguard harvested rainwater quality in rural domestic households. " Journal Of Water & Health 8(2): 334-345, 2010
15. Kotlarz, N. , Lantagne, D. S., Preston, K. , Jellison, K. . "Turbidity and chlorine demand reduction using locally available physical water clarification mechanisms before household chlorination in developing countries" Journal Of Water & Health 7(3): 497-506, 2009
16. Mannapperuma, W. K., Abayasekara, C. L., Herath, G. B., Werellagama, D. B., Heinonen-Tanski, H. H.. "Comparison of Bacteriological Methods for Detecting Enumerating Total Coliforms and Escherichia coli in Water" Research Journal of Microbiology 7(3): 497-506, 2009
17. Mwabi, J. , Mamba, B. , Momba, M. . "Removal of Escherichia coli and faecal coliforms from surface water and groundwater by household water treatment devices/systems: a sustainable solution for improving water quality in rural communities of the Southern African development community region" International Journal Of Environmental Research And Public Health 9(1): 139-170, 2012
18. Ordonez, A. L., Gomez, J. D., Cudmani, N. M., Vattuone, M. A., Lsla, M. I.. "Antimicrobial Activity of Nine Extracts of Sechium edule (Jacq.) Swartz" Microbial Ecology In Health & Disease 15(1): 33, 2003
19. Preston, K. , Lantagne, D. S., Kotlarz, N. . "Turbidity and chlorine demand reduction using alum and moringa flocculation before household chlorination in developing countries" Water Health 8(1): 60-70, 2010
20. (2012, November 30). Agta, Casiguran Dumagat. Ethnic Groups of the Philippines. . Retrieved from: http://www.ethnicgroupsphilippines.com/people/ethnic-groups-in-the-%20philippines/agta-casiguran-dumagat/
21. (). Options for Pre-treating Water in Chlorination Projects. Centers for Disease Control and Prevention. . Retrieved from: http://www.cdc.gov/safewater/chlorination-pretreatment.html
22. Peralta, J. T.. (2011, November 30). Casiguran Agta / Casiguran Dumagat. Aurora.ph. . Retrieved from: http://www.aurora.ph/baler-aurora-book/agta-dumagat.html

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